Nuclear Magnetic Resonance

Tools of structural biology

Chemistry molecule by molecule

Nuclear Magnetic Resonance (NMR) is a physical phenomenon in which certain atomic nuclei absorb and re-emit electromagnetic radiation when placed in a strong magnetic field. Various NMR spectroscopies can be collected by hitting samples with a train of pulses with radio frequency. The data gathered enables scientists to determine the structure and dynamics of molecules, as well as the interaction of different molecules.

A protein called tau, if spliced incorrectly, can cause diseases like Alzheimer's. Here, an NMR study done for Disney lab at UF Scripps reveals structural insights that helped the group develop potential medicines for tau-related diseases. 10.1021/jacs.0c00768

Resources and Equipment

The core has four Bruker nuclear magnetic resonance instruments:

• Bruker AVANCE III 700 MHz instruments with QCI Cryoprobe.
• Bruker AVANCE NEO 600 MHz instruments with TCI Cryoprobe and SampleCase
• Bruker AVANCE NEO 400 MHz instruments with SampleXpress
• Bruker AVANCE 400 MHz instruments with SampleXpress

Those instruments perform all sorts of multi-dimension NMR experiments, and detect a variety of nuclei.

By connecting these highly sensitive instruments to the internet via a proprietary Scripps Florida server, our scientists and their collaborators can access the collected data from their office or laboratory.

Services

• Training
• Various experiments set-up
• Data collection and analysis

While priority of use is always given to Scripps Research Florida researchers, time on the instruments is also made available on a fee-for-service basis to outside users.
(Subject to change; call for current pricing and quotes)

Fee-for-Service Pricing

All spectra are run by Scripps Florida personnel, and results are accessible to outside customers by a proprietary Internet link.

•     Time on 400 MHz instrument: $60/hour
•     Time on 600/700 MHz instrument: $120/hour

Contact