Microscopes: SP8 Multiphoton
Advanced Light Microscopy Core

Leica Upright SP8 Confocal with Pulsed Chameleon Laser, motorized stage and water immersion objectives. There are only 2 upright microscope frames in the core. This one is great for electrophysiology experiments due to the depth provided by the Chameleon Laser.

Objectives:
10X NA 0.40 HCXPL APOCS
L25X .95NA water immersion
63X 1.20 NA
40X 1.10 HCPL APO CS2

Lasers:
Coherent Chameleon 2P Laser – This laser can be tuned to wavelengths from 680 to 1080. The emission wavelength is approximately half the excitation wavelength.
442 Diode – CFP
Argon – YFP, GFP (488)
DPSS 561 – Rhodamine or Texas Red
HeNe 633 – Cy5

Reservation guidelines

Weekday, daytime appointments:

  • Appointments should end before 1 p.m. or begin after 1 p.m.
  • Exception: if one user signs up for a morning slot and another has an afternoon slot, a third user may fill in the time between the existing appointments.
  • Each user may only sign up for one day time appointment per week day.
  • Reservations may be made up to seven days in advance.

Evening (after 7 p.m.) and weekend appointments

  • No restrictions on time.
  • May be made up to one month in advance.
  • Can be combined with other appointments (e.g., a user can book 7 a.m. – 1 p.m. and 7 p.m.0– 10 p.m. on the same day)

Additional notes:

Any unused appointments must be canceled in advance

If a user needs an exception to this scheduling for a specific experimental reason, exceptions can be made.