cores and resources
Visualizing Life at the Scale of Nanometers
Laser, light, confocal dStorm super-resolution, and multiphoton microscopes are available for the use of the scientific community.
Getting Started
Welcome to the Advanced Light Microscopy Core. We’re pleased to help you obtain the training and experience you will need to successfully use our various laser, confocal and super-resolution microscopes for your research. Please allow yourself time to learn to use the microscopes. A preliminary training session along with one user session demonstrating the ability to use the microscopes competently is required to become a registered user.
Alex Velong
Scheduling and Training
Start by contacting Alex Velong. His office is located at 120 Scripps Way, Building B, Room 213. Office phone and email are 954-292-3391, alexvelong@ufl.edu.
Next, you will be sent a calendar invitation. Note that each machine requires its own, separate training session. Once you’ve been trained and demonstrated competency on a given microscope, you will be considered a registered user.
Important: Before every session, always used the sign-in sheet with your username and the name of your PI, for billing purposes.
Please clean objectives after use with available alcohol and swabs.
Available Microscopes
Please click on detailed information to receive a list of objectives, filters, application information, and images created by the individual microscopes.
FV3000 Confocal Microscope with Abbelight STORM C296C
Confocal Microscope with the Abbelight 180 3D dStorm TIRF module. The FV3000 is a spectral confocal system with 4 detectors and a resonant scanner. It has CellSens analysis package with a Trusight Constrained Iterative Deconvolution Module.
SP8 Multiphoton
Leica Upright SP8 Confocal with Pulsed Chameleon Laser, motorized stage and water immersion objectives. There are only 2 upright microscope frames in the core. This one is great for electrophysiology experiments due to the depth provided by the Chameleon Laser.
FV1000 B137 E
FV1000 Building B Room 137EFV1000 confocal IX81 Microscope is an inverted microscope with a heating chamber and CO2.
FV1000 B137E Detailed Information
FV1000 B137F
This microscope can perform fixed tissue applications with 1.5 corrected coverslips.
FV1000 B137F Detailed Information
Zeiss Elyra SIM Microscope
Axio Observer Inverted microscope platform with Elyra Structured Illumination System. This microscope has an automated stage and is capable of doing PALM and SIM microscopy.
Zeiss Elyra Detailed Information
ALBA v5 FRET / FLIM Microscope
A Nikon inverted microscopy system used for confocal, FastFLIM, FFS,
Scanning-FCS, and single molecule measurements.
IX81 Spinning Disk Confocal
An Olympus inverted microscope with a spinning disk. This microscope has a Lumencor Celesta Quattro LED illumination system with deconvolution and a motorized stage.
IX81 Spinning Disk Detailed Information
VS120 Slide Scanner
An upright automated slide scanner with 100 slide capacity. This microscope allows for excellent stitching with z-stack capabilities. This is for both fluorescence and brightfield imaging.
Zeiss Discovery V-8 Stereo Microscope
Stereo dissecting microscope with GFP/ RFP fluorescence and a transmitted light base. 1X and 1.5 X objectives.
Pricing (In Dollars)
| iNSTRUMENT | iNTERNAL | eXTERNAL NOT-FOR-PROFIT | eXTERNAL fOR-PROFIT |
|---|---|---|---|
| FV3000 Confocal Microscope with Abbelight STORM | |||
| Self Service | 30 | 57.96 | 86.94 |
| Assisted | 30 | 57.96 | 86.94 |
| Training | 45 | 86.94 | 130.41 |
| Zeiss Elyra Axio Observer | –– | –– | –– |
| Self Service | 25 | 48.30 | 72.45 |
| Assisted | 25 | 48.30 | 72.45 |
| Training | 35 | 67.62 | 101.43 |
| Alba v5 Microscope System | –– | –– | |
| Self Service | 25 | 48.30 | 72.45 |