Advanced Light Microscopy

cores and technologies

Visualizing Life at the Scale of Nanometers

Laser, light, confocal dStorm super-resolution, and multiphoton microscopes are available for the use of the scientific community.

Disease-causing huntingtin, shown in red, interacts with ribosomes, shown in green, in a striatal neuron. The nucleus is blue. Courtesy Subramaniam lab.

Getting Started

Welcome to the Advanced Light Microscopy Core. We’re pleased to help you obtain the training and experience you will need to successfully use our various laser, confocal and super-resolution microscopes for your research. Please allow yourself time to learn to use the microscopes. A preliminary training session along with one user session demonstrating the ability to use the microscopes competently is required to become a registered user.

Alex Velong

Advanced Light Microscopy Core Manager
Phone: (561) 228-3456

Scheduling and Training

Start by contacting Alex Velong. His office is located at 120 Scripps Way, Building B, Room 213. Office phone and email are 954-292-3391, alexvelong@ufl.edu.

Next, you will be send a calendar invitation. Note that each machine requires its own, separate training session. Once you’ve been trained and demonstrated competency on a given microscope, you will be considered a registered user.

Important: Before every session, always used the sign-in sheet with your username and the name of your PI, for billing purposes.

Please clean objectives after use with available alcohol and swabs.

Available Microscopes

FV1000 B137 E

FV1000 Building B Room 137EFV1000 confocal IX81 Microscope is an inverted microscope with a heating chamberand CO2.

This microscope can perform fixed tissue slide applications and live cell applications.

It has a manual stage so it lacks the ability to stitch. It’s not considered high speed but can image movies and timelapse. The objectives require a 1.5 coverslipIt has 405, 488, 515, 543, and 635 lasers, a manual stage and DIC optics. This images DAPI, GFP, TRITC Rhodamine, mCherry, CY5Optics:4XSAPO (NA 0.16, WD 13MM)20X SAPO (NA 0.75, WD 0.60MM)40X FL (NA 1.30, WD 0.2MM)60XNAPO (NA 1.42, WD 0.15MM)

FV3000 Abbelight C296C

The institute has acquired a HybridLaser Confocal Microscope with the Abbelight 180 3D dStorm TIRF module. The FV3000 is a true spectral confocal system with 405, 488, 561, 640 laser excitation. Two Cooled GaAsP PMT detectors allow the simultaneous imaging of two laser channels and a transmitted light channel at the same time. The confocal is capable of resonance scanning at high speeds! It also has a Trusight Deconvolution Module. The optics are allApo corrected and include a 4X, 10X, 20X, 40X oil NA 1.4, 60X oil High Resolution TIRF Oil Objective NA 1.50, and a 100X Oil High Resolution TIRF objective N.A. 1.5.All the included optics have Nomarski components to aid in the location of structures without submitting the sample to fluorescence excitation. 

This microscope has a motorized stage and excellent stitching capabilities. It also has an excellent array of stagetop incubation chambers to aid in live cell imaging. There is a TruFocus Z DriftCompensation module that will maintain focus for time lapse experiments.An Abbelight 180 SAFE dSTORM TIRF module is connected to the left side imagingport of this microscope further expanding the capabilities to allow it to image with SuperResolution technology.

Single-molecule localization precision down to 10 nm × 10 nm × 15 nm (X×Y×Z)Nearly 10 times higher resolution than with standard confocal systems Large field of view of up to 150 x 150μm at 100x. The system also has single color TIRF capabilities.

SP8 Multiphoton

Reservation guidelines

Weekday, daytime appointments:

  • Appointments should end before 1 p.m. or begin after 1 p.m.
  • Exception: if one user signs up for a morning slot and another has an afternoon slot, a third user may fill in the time between the existing appointments.
  • Each user may only sign up for one day time appointment per week day.
  • Reservations may be made up to seven days in advance.

Evening (after 7 p.m.) and weekend appointments

  • No restrictions on time.
  • May be made up to one month in advance.
  • Can be combined with other appointments (e.g., a user can book 7 a.m. – 1 p.m. and 7 p.m.0– 10 p.m. on the same day)

Additional notes:

Any unused appointments must be canceled in advance

If a user needs an exception to this scheduling for a specific experimental reason, exceptions can be made.